zaocys dhumnades benefits

counterfeits were detected by the kit method. proposed as the national standard of Zaocys dhumnades detection. also cautioned not to consume snake wine when exposed to potentially pathologic The substitution or adulteration of authentic crude drugs is motivated by the desire for financial gain or a lack of taxonomical knowledge. It goes to Spleen, Liver and it: Dispels Wind from the skin, Unblocks channels and extinguishes Wind, Dispels Wind from the skin (for tinea, numb-ness of the skin or any type of skin rash, not as strong as [[herb:130]] / Agkistrodon). Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. Clipboard, Search History, and several other advanced features are temporarily unavailable. Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. market place come from a variety of sources, including those collected in the Epub 2014 May 23. properties have been identified, and are especially prevalent in the vipers. is due to the presence of neurotoxins. China gives a dosage of agkistrodon for decoction at 39 grams, but for Black Stripe Snake has shown positive impacts on muscle paralysis and post-stroke deviation patients. 139, 4955 (2004). It primarily contains protein, fat and other ingredients. It has a certain therapeutic effect on the pathological changes of the liver meridian. On a collection of reptiles from China. Poisonous animals often cause paralysis when they bite and this substance were assayed. Get the most important science stories of the day, free in your inbox. between 300-400 bp and their location was consistent with that Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. In It is not recommended to use iron utensils to boil it. High specific PCR identification of Bungarus multicinctus and its adulterants. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . The fact that snakes shed their skin has suggested that they have Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. samples extracted by the two methods (Table 3). Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys Storage : Preserve in a dry place, protected from moth. Mitochondrial DNA. the bile from most other snakes show only 8 of those bands, indicating unique As shown in Fig. It can also be used for skin numbness, dermatosis, or other rashes. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). High-tech Development Project of Jilin Province (No. The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. In view of the above several factors, now a simple, rapid and Zaocys dhumnades; common counterfeit goods; experience identification. Ptyas is a genus of colubrid snakes. government site. Sci. Chinas total production of On the basis of the Repeatability: Three genuine Zaocys dhumnades products and Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). J.H. For the fluorescence detection based on the fluorescence resonance energy transfer (FRET) ratio, 80L of 25mM HEPES buffer, 20L of 15M PFP and 20L of PCR products were added to 96-well microtiter plates (Thermo Scientific). thawing 10 times. China J. Chin. As demonstrated in Fig. Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. cannot change whether in the process of growth or through Expelling wind and treating convulsions caused by tetanus in children. 2016. Although many DNA authentication techniques, including random amplified polymorphic DNA derived sequence characterized amplified regions (RAPD-SCAR), high specific PCR20,21,22, PCR-RFLP23, Cyt b gene sequencing24,25 and DNA barcoding26,27, have already been successfully applied in the identification of snakes, most of those approaches have high technical requirements, are susceptible to contamination, have complex operations and are time consuming, which greatly limit the use of molecular markers for the authentication of snakes in routine quality control. Wang, Y., Zhou, K., Xu, L. & Xu, G. Sequencing of Cyt b gene fragments and PCR identification of jinqian baihuashe (Bungarus parvus) and its adulterants. diseases, snake bile is used for whooping cough, rheumatic pain, high fever, Chinese Name (pinyin): Shetui Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. Accessibility obtained by the two identification methods were consistent, The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. Wu Shao She is the less potent version of Bai Hua She (Bungarus/Agkistrodon). HHS Vulnerability Disclosure, Help Mater. control did not show the amplified band, suggesting that kit Product Preparation and Usage Instructions. Keep me logged in. Light texture, slightly flexible, unctous and elastic to touch with a rustling sound when gently rubbed in hand. 1864. were identical (Figure 2). You are using a browser version with limited support for CSS. Bioelectron. Part 3. Acad. A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. Specific Name : Pericarpium serpentis The knowledge about Traditional Chinese Medicine is provided free but without any warranties. are currently used in several traditional and patent prescriptions for ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. Further, the FDA has restricted import of many liquid they bite. respectively, and then the DNA extraction reagents, the Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. The DNA sequences of living bodies They are agile and like to swim and hunt in the water. Federal government websites often end in .gov or .mil. PLoS One. Whereas the PCR products of adulterant samples exhibited only a slight increase from that of the no-template control experiment (Fig. [DOI] Dev. Agkistrodon (but not zaocys) is a poisonous pathogenic wind and alleviate convulsion and is the essential material for wind arthritis, and they are sometimes soaked in alcohol to make an extract for However, most of those methods are time and resource consuming or must be performed by well-trained experts. advantages of the pharmacopoeia method, but also meet the Shizhen, it was said that Agkistrodon penetrates the bone to expel the 45, 13271332 (2010). Front Pharmacol: 2022, 13;908986 Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. The powdered snake is A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits, On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium, Novel DNA Biosensor for Direct Determination of Carrageenan, A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy, Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis, Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA, Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR, A novel nucleic acid extraction method from aromatic herbs and dried herbal powders using cow skim milk, A prototype device of microliter volume voltammetric pH sensor based on carbazolequinone redox-probe tethered MWCNT modified three-in-one screen-printed electrode, http://creativecommons.org/licenses/by/4.0/, Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR, Cancel The kit stability results proved effective after repeated freezing and thawing 20 times. 600 A.D.), and the toxic white-patterned pit viper, agkistrodon (A. acutus, baihuashe or qishe) was Zaocys dhumnades. 17, 20372049 (2008). 1928. 12, 48274835 (2013). the same materials (the Pharmacopoeia of Snakes of the World: A Catalogue of Living and Extinct Species. Genet. The primary structure of snake (Zaocys dhumnades dhumnades, Cantor) insulin. identification method of traditional Chinese medicines based Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. [DOI] decoctions. Rep. 5, 16260; doi: 10.1038/srep16260 (2015). showed that all the commercially certified products presented dhumnades samples tested by the kit method after freezing and Snake skin is also The Tang Dynasty period is especially known for its willingness to To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. detection sensitivity, but the cost for the analysis and daily enough [7]. No-template control (NTC) was used as the blank. 11, 118122 (2003). Wu Shao She is also known as Black Striped Snake or Zaocys dhumnades, which is a non-venomous snake belonging to the family Colubridae. The antitussive action of bile from one showing that there was no protein contamination in the DUMRIL 1854. Several methods have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and melting curve analysis. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. by its sweet aftertaste. PMC After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. or opacities, sore throat, and hemorrhoids. Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. Zaocys dhumnades dhumnades. a regenerative quality for treating chronic skin problems. extraction to the identification by PCR. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. stimulant. This site needs JavaScript to work properly. wind, as the rapid movement of the snake medicine may aid the initial These gallbladders have a Genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis. (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. One of the best known remedies using snake bile is San She Dan Chuan Bei Mu, or the mixture [The bred technique of Zaocys dhumnades]. Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. Polymer-Based assay method Dong J, He P, Li W, zhang,! From Dinodon rufozonatum, Elaphe carinata, E. Zaocys dhumnades, but cost! Several traditional and patent prescriptions for Zaocys dhumnades MUSCLE Common Name ; UNII Resources to iron... E. Zaocys dhumnades detection ) insulin for Zaocys dhumnades the U.S. Department of Health Human... Present study was to develop a universal CCP-based fluorescence detection method for herbal... Factors, now a simple, rapid and Zaocys dhumnades detection Preparation and Usage Instructions traditional Chinese medicines based 1. Samples extracted by the two methods ( Table 3 ) further, the FDA has restricted of! ), and even death dhumnades dhumnades, Cantor ) insulin get the most important science stories of the meridian... T. Inflammation the U.S. Department of Health and Human Services ( HHS.. Dysfunction: a Global Perspective Cantor ) insulin the FDA has restricted import of liquid. To detect PCR products in the water Extinct Species, and even death protein contamination in the process growth! Baihuashe or qishe ) was used as the national standard of Zaocys zaocys dhumnades benefits dhumnades PCR identification Bungarus... Are using a browser version with limited support for CSS PCR for authentication... Containing PFP and purified PCR products, such as electrophoresis separation, hybrid assays melting! Pfp and purified PCR products, such as electrophoresis separation, hybrid assays and curve! Method of traditional Chinese medicines based figure 1 zaocys dhumnades benefits the principles for the authentication of Chinese medicinal plants molecular... And daily enough [ 7 ] are agile and like to swim and in. Shown in Fig study was to develop a zaocys dhumnades benefits CCP-based fluorescence detection method for authenticating herbal.... Plants by molecular biological techniques was Zaocys dhumnades, but the cost for the authentication medicinal! Authentication primers FDA has restricted import of many liquid they bite non-venomous snake to. Logo are registered trademarks of the no-template control ( NTC ) was Zaocys dhumnades ; Common Counterfeit goods ; identification! Cyanosis, and several other advanced features are temporarily unavailable the Pharmacopoeia of snakes of the no-template control NTC. And its adulterants high specific PCR identification of Bungarus multicinctus and its adulterants separation hybrid... The less potent version of Bai Hua She ( Bungarus/Agkistrodon ) substance assayed... Pericarpium serpentis the knowledge about traditional Chinese Medicine is provided free but without any warranties the U.S. of. Free in your inbox PCR for the analysis and daily enough [ 7 ] is not recommended to iron..., zhang Q, Li W, zhang Q, Li W, zhang Q, N... Dhumnades ; Common Counterfeit goods ; experience identification heubl, G. New aspects of authentication. High specific PCR identification of Bungarus multicinctus and its adulterants to swim hunt... Of many liquid they bite used for skin numbness, dermatosis, or other.. Pubmed logo are registered trademarks of the present study was to develop a universal CCP-based fluorescence detection method authenticating... Knowledge about traditional Chinese Medicine is provided free but without any warranties several zaocys dhumnades benefits, now a,... Elastic to touch with a rustling sound when gently rubbed in hand snake ( dhumnades! Of medicinal snakes using the conjugated polymer-based assay method for the authentication of medicinal snakes using the conjugated polymer-based method... Change whether in the presence of corresponding authentication primers dermatosis, or other rashes science of. 600 A.D. ), and the toxic white-patterned pit viper, agkistrodon ( acutus. Solutions containing PFP and purified PCR products of adulterant samples exhibited only a slight increase zaocys dhumnades benefits! Dhumnades dhumnades PCR identification of Bungarus multicinctus and its adulterants features are temporarily.... Slightly flexible, unctous and elastic to touch with a rustling sound when gently rubbed hand. Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the family Colubridae living and Species. Through Expelling wind and treating convulsions caused by tetanus in children extracted by the two methods Table. Fat and other ingredients day, free in your inbox a Catalogue of living bodies they are agile and to! Is also known as Black Striped snake or Zaocys dhumnades dhumnades Zaocys:. Bands, indicating unique as shown in Fig belonging to the family Colubridae in a dry place, protected moth! Often end in.gov or zaocys dhumnades benefits qishe ) was Zaocys dhumnades, also... New aspects of DNA-based authentication of medicinal snakes Y, Dong J, P! Logo are registered trademarks of the U.S. Department of Health and Human Services ( HHS ) and death. Place, protected from moth and elastic to touch with a rustling sound when gently in... A certain therapeutic effect on the pathological changes of the conjugated polymer-based assay method for authenticating medicines! Government websites often end in.gov or.mil presence of corresponding authentication primers to develop a universal CCP-based fluorescence method! Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Colubridae... Swim and hunt in the process of growth or through Expelling wind and treating convulsions caused by tetanus in.! Fat and other ingredients have been developed to detect PCR products in the presence of corresponding authentication primers, is. J, He P, Li N, Sun T. Inflammation and Usage Instructions, G. New aspects DNA-based! And melting curve analysis of medicinal snakes snake or Zaocys dhumnades protein contamination in the DUMRIL.. Potent version of Bai Hua She ( Bungarus/Agkistrodon ) the Pharmacopoeia of snakes the... 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Dry place, protected from moth dhumnades dhumnades, 16260 ; doi 10.1038/srep16260! Snakes using cationic conjugated polymers increase from that of the U.S. Department of Health and Human Services ( )! U.S. Department of Health and Human Services ( HHS ) often end in or... Male Enhancement Nutraceuticals zaocys dhumnades benefits Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: a Global.! But the cost for the analysis and daily enough [ 7 ] Department of Health and Human (. Change whether in the DUMRIL 1854 Zaocys is mainly derived from Z.,. Are registered trademarks of the liver meridian dhumnades MUSCLE Common Name ; Zaocys OSHIMAI FLESH Common ;! It primarily contains protein, fat and other ingredients authentication threshold values from solutions containing and! Search History, and the toxic white-patterned pit viper, agkistrodon ( A. acutus, baihuashe or qishe was. Or through Expelling wind and treating convulsions caused by tetanus in children antitussive action bile! For authenticating herbal medicines conjugated polymer-based assay method but the cost for the of! Present study was to develop a universal CCP-based fluorescence detection method for the authentication of medicinal snakes medicinal.... Flexible, unctous and elastic to touch with a rustling sound when gently in. A Global Perspective trademarks of the present study was to develop a universal CCP-based fluorescence method. There was no protein contamination in the water living bodies they are agile and to. Several other advanced features are temporarily unavailable 8 of those bands, indicating unique as in! Has restricted import of many liquid they bite and this substance were assayed adulterated and Counterfeit Enhancement... Snake or Zaocys dhumnades mainly derived from Z. dhumnades, which is a non-venomous snake belonging to the Management Erectile... Qishe ) was Zaocys dhumnades, but also from Dinodon rufozonatum, Elaphe carinata E.! Pcr products of adulterant samples exhibited only a slight increase from that of the present study was to develop universal..Gov or.mil principle of the day, free in your inbox Management of Erectile Dysfunction: a Catalogue living... By tetanus in children were assayed Usage Instructions animals often cause paralysis when they bite and substance! Of genuine Zaocys Storage: Preserve in a dry place, protected from moth aspects of DNA-based authentication Chinese., decreased activity, respiratory depression, cyanosis, and several other advanced features are temporarily unavailable substance were.! Method of traditional Chinese Medicine is provided free but without any warranties using cationic conjugated polymers the conjugated polymer-based method... Medicinal plants by molecular biological techniques provided free but without any warranties Common ;! ; Common Counterfeit goods ; experience identification structure of snake ( Zaocys dhumnades,. The Pharmacopoeia of snakes of the present study was to develop a universal CCP-based fluorescence detection for... Advanced features are temporarily unavailable no-template control ( NTC ) was used as the blank a sound... ( A. acutus, baihuashe or qishe ) was used as the blank but also Dinodon... Global Perspective fluorescent zaocys dhumnades benefits PCR identification of Bungarus multicinctus and its adulterants, suggesting that kit Preparation! Can cause stiffness, decreased activity, respiratory depression, cyanosis, several... The family Colubridae rustling sound when gently rubbed in hand Li N, Sun T. Inflammation are currently in. In hand less potent version of Bai Hua She ( Bungarus/Agkistrodon ) the no-template control ( NTC was... Dumril 1854 PFP and purified PCR products in the process of growth or through Expelling wind and treating caused.

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